Research Projects
July 31, 2024 2025-07-31 17:21Research Projects
Research Projects
Epizoothological investigations on spread of pox in partridges in Bulgaria
Epizoothological investigations on spread of pox in partridges in Bulgaria
Project title: Epizoothological investigations on spread of pox in partridges in Bulgaria.
Key words: partridges (Perdix perdix), avian pox, epizoothology
Investigations on tuberculosis in badger (Meles meles) for monitoring of the disease in bases for intensive breeding of red deer in our country
Investigations on tuberculosis in badger (Meles meles) for monitoring of the disease in bases for intensive breeding of red deer in our country
Project title: Investigations on tuberculosis in badger (Meles meles) for monitoring of the disease in bases for intensive breeding of red deer in our country.
Key words: tuberculosis, badger (Meles meles).
Virological and serological investigations on pox in partridges in Bulgaria
Epizoothological investigations on spread of pox in partridges in Bulgaria
Project title: Virological and serological investigations on pox in partridges in Bulgaria
Key words: partridges (Perdix perdix), avian pox, virus isolation, immunization, cross protection.
Epizoothological investigations on spread of pox in partridges in Bulgaria
Virus-induced chicken hepatoma – a model for investigations on cancerogenesis and antineoplastic activities of metal compounds
Summary
The LSCC-SF(Mc29) permanent cell line established from a transplantable chicken nepatoma induced by the myelocytomatosis virus Mc29, was cloned and four sublines were obtained – Е7, Е10, D6E10, G9B4. It was found in our previous investigations (Grant CC 1/2000) that these cells differed significantly in morphology, karyotype, proviral load and tumour growth in vivo. The aim of the project presented here is to study comparatively the expression of some tumour associated antigen/s/ (TAA/s/) and tumour suppressor genes (p53) in the experimental model. Hybridoma cells will be developed and monoclonal antibodies (mAbs) obtained will be characterized and used for identifying the TAA/s/ in hepatoma cells. P53 will be evaluated by immunological (usim\ng commercial antibodies) and molecular techniques (PCR, sequencing). Some other avian cells transformed by the myelocytomatosis viruses Mc29 and Mc31 will be included in some of the experiments for comparative investigations.
Cytotoxic and antiproliferative activities of newly synthesyzed metalloporfirins and complexes of Cu(II), Co(II), Zn(II), Ni(II), V(IV), Pt(II), Pd(II) and Ru(II) complexes with different ligands (Schiff and Mannich bases and their derivatives, biguanides, aminoacids) on LSCC-SF(Mc29) cells will be compared to that of some of tumour and nontumour cells from chicken, mouse, rat and human origin. In order to study better the biological activities of metal complexes their antimicrobial effects in vitro will be also studied.
Key words: transplantable chicken nepatoma induced by the myelocytomatosis virus Mc29, tumour associated antigens, monoclonal antibodies, protein p53, cytotoxic/antiproliferative activity, metal compounds/metalloporfirins, cell lines, antimicrobial activity.
Following of the surviving of pathogenic micro-organisms in poultry litter in periodic-adding process of methane fermentation and comparative assessment in relation to the decontamination in other methods of bioprocessing
Following of the surviving of pathogenic micro-organisms in poultry litter in periodic-adding process of methane fermentation and comparative assessment in relation to the decontamination in other methods of bioprocessing
Scientific group: Assoc. prof. Teodora P. Popova (FVM, FTU), Dr. Iosko P. Petkov (FVM, FTU), Prof. Bayko D. Baykov (FTU and NBU), Assoc. prof. Ivan S. Simeonov – (IM, BAN), Ionko N. Nakov (student, FVM, FTU), Plamen D. Kiosev (student, FVM, FTU)
Summary
Microbiological investigations, connected with tracing of the changes in the quantities of the bacteria in bioreactors with periodic-addingand unbroken mesophilic regimes of anaerobic removing its fence of water solutions of litter from broiler chickens have been carried out. Marked strains of Esherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, multiresistant to amphenicols and tetracyclines, have been introduced in the bioreactors in definite quantities. In order to establish the quantities of the marked and not marked microorganisms, a samples from the exits (the last bioslames) have been taken at intervals of 3 – 4 days to the ends of two compared processes. In the both regimes of anaerobic removing its fence of water solution of poultry litter, gradually diminution of the contents of the marked strains has been established. The reduction was most fast at the staphylococci and most slowly at P. aeruginosa.
Moreover comparative investigations of the efficacy of two methods for treatment of litter from broiler chickens and of cattle manure have been performed in order with a view to their decontamination. The experiments have been done in ordinary composts and after treatment with CaO. For that purpose marked strains of E. coli, P. aeruginosa and S. aureus, multiresistant to amphenicols and tetracyclines, have been introduced in the manures in definite quantities at the initial stages of treatment. For establishment of the quantities of the marked and not marked microorganisms, a samples from the three variants of the composts have been taken in a week intervals.
For the isolation and cultivation of the marked bacteria selective media have been used, which contained definite wide-spectrum antibiotics in suitable concentrations: Eosin Methylene Blue agar for E. coli, Cetrimide аgar for P. aeruginosa and Chapman Stone agar for S. aureus. In order to determine the general quantity of aerobic and facultative anaerobic bacteria Mueller Hinton’s agar without antibiotics has been used. The quantitative determination of the microorganisms has been accomplished by the classical method of serial tenfold increasing dilutions of the investigated materials in sterile physiological solution. Their sowings on the selected media with and without antibiotics have been performed threefold for each medium and dilution. After incubations in 37оС for 24-48 h the average number of the developed colonies has been determined and the quantity of the colony-forming units (CFU) in 1 mL of the initial material has been calculated.
At the periodic-addingmesophilic regime of anaerobic removing its fence, which flows in 45 days, the three marked strains were introduced in bioreactor each by 107CFU/g of all its content. After the fourth week from the beginning of the process marked staphylococci did not been established in the bioshlam. The marked E. coli disappeared after the fifth week, but P. aeruginosa have been revealed in minimal values till the end of the investigation. However, after the introducing of the bioshlame in the environment, single bacteria of that species will fall in unit of area in quantities which could not be able to provoke infections and to represent ecological danger. Their viability in the soils is not high, especially at low humidity and influence of the ultraviolet sun rays. That gives a reason the final bioshlame from the periodic-addingmesophilic regime to be evaluated as epizoothologically safe for introducing in the soils.
In the unbroken regime of anaerobic removing its fence, which flows in 22 days, the three marked strains were introduced in bioreactor each by 5.104CFU/g of all its content. In that concentrations they disappeared in shorter period (during 2 – 3 weeks).
In the composts of litter from broiler chickens the marked bacteria have been added each by 105CFU/g of all content. In the compost of cattle manure the same bacteria were introduced in double smaller concentrations. Behind the treatment with CaO the marked microorganisms died during about 2 – 3 weeks. After three weeks these bacteria did not established in ordinary composts too. Only in the poultry litter P. aeruginosa has been detected in little quantities through the fourth week, but later on disappeared from the compost.
The results from our investigations confirmed high efficacy of the treatment with CaO in order to fully decontamination of the poultry litter in short time. The ordinary compost was efficacy in this respect too, but by that method fully decontamination of the poultry litter achieved in a longer period of time. The last bioslame from the unbroken regime of anaerobic removing its fence was fully free from pathogenic bacteria too.
Key words: anaerobic removing its fence, periodic-adding process of methane fermentation, mesophilic regime, composts, marked bacteria.
Studies on the parasitocidic impact on the hydrodynamic effect on the sexual products of parasites with economical importance for Bulgaria
Studies on the parasitocidic impact on the hydrodynamic effect on the sexual products of parasites with economical importance for Bulgaria
The ways of infection of animals and humans with parasites are diverse. One of the ways most often is per os infection, which happens through pod and water. Water is permanently necessary for the existence of humans and animals and plays a very important role in episootology and epidemiology of a number of parasitic diseases, because the infection with some parasites is realized through the water. There is even such parasites, which undergo one part of their development in water and their biological cycle cannot be finished without it. Some of them release third-stage larvae in the water.
The applied methods of purification of water through chlorination send other means do not make harmless. The water as regards eggs and larvae of helminthes and oocysts of eimeria.
Our preliminary investigations show that the hydrodynamic effect in water medium, produced with the help of hydrodynamic generator (HD-2) and being result of occurance of physical phenomena, leads to full destruction of the larvae and eggs of some parasites.
Our observations show that the occurred forces in water medium, where the larvae are put in lead to disintegration of their bodies. The same injuries are observed as well in treatment of fertile eggs of equid strongylates in result of which those eggs do not develop.
The above gives us a reason by this project to carry out detailed investigation of the influence of this effect on the sexual products of parasites with economic importance in our country.
On the basis of the obtained results from the investigation should be prepared regulations for treatment of drinking water and technological water with purpose of purification from sexual products of helminthes and protozoa.
